Doctors in a hospital in Ajdabiya told the BBC that three medical students among the dead were.
NATO said that it was in the incident in search but that it was very difficult, check information about what happened.
The attack came after rebels allegedly a flak thrown.
In the meantime one Libya's Government truce rebel offer rejected. Spokesman Moussa Ibrahim dismissed the idea as "mad".
Col Muammar Gaddafi loyal troops never held cities would withdraw by the rebels, who were besieged, he said.
He condemned also the last coalition air strikes as a "Crime against humanity" and had it civilian casualties in an attack on Thursday said.
Vehicles destroyedThe rebels were on the way to Brega, if they are thrown in the air with a flak, reported BBC Nick Springate from the scene of the attack.
The rebel convoy was on its way to the front in the vicinity of Brega, when the attack took placeThe way to the front with holes caused by what a plane NATO A10 looks like interspersed, says our correspondent.
The remains of four pick-up trucks and an ambulance, as well as the graves of which in the attack are killed on both sides of the street he adds.
The attack is assumed, that happened between 2300 and midnight according to local time. There is very little clarity around the incident, but can have a flak were released to celebrate, our correspondent says.
A coalition level then fire on the convoy, destroy the five vehicles, opened the said rebels.
NATO said it regretted the loss of civilian life.
"Nato aircraft in the sky over Libya are, because they are no fly zone, the UN mandate for the protection of civilians, enforcement" said Oana Lungescu of the BBC.
NATO spokeswoman Oana Lungescu: ' we take all possible care to ensure that no lives lost due to NATO strikes 'But she added: "If someone against our aircraft raised she will have the right to respond, they enforce a no fly zone." "All flak would against that Act be."
On Friday, the head of the rebel transitional explains National Council, Abdul Jalil Ibrahim, such as a ceasefire agreement may after a meeting with UN Special Envoy A. al-Khatib in the eastern city of Benghazi.
"We have no objection to a cease-fire but under the condition that Libyans have full freedom in Western cities in to express their views, and also, that the forces that have besieged the cities to withdraw," he told reporters.
"Our main demand is the departure of Muammar Al-Qadhafi and his sons from Libya." "This is to a claim, we will go back."
Mr Abdul Jalil, who as Justice Minister in protest against the use of force against the demonstrators to stop, also said he believed that the Coalition will begin arming the rebels despite the UN arms embargo against Libya.
The Libyan Government spokesman, Moussa Ibrahim, said the rebel Transitional National Council demands were "impossible""We think that if the international community want to protect civilians in accordance with the international resolutions, they should take the appropriate measures which include the type to go ahead arm the rebels," he added.
Moussa Ibrahim dismissed the offer of the conditional cease-fire, the rebels describe as "tribal, violent, with no unified leadership, al Qaeda links."
"The rebels peace offered never." They offer no rest. "You make impossible demands."
"This is not crazy, not what I know is", he added. "We will not leave our cities." "We are the Government, not them."
Mr Ibrahim also said that through an "immoral" air strike in the Eastern village of Zawia el Argobe, 15 km (9 miles) of Brega were killed six civilians.
He told reporters in Tripoli "some mad and criminal Prime Minister and President of the Europe lead a crusade against an Arab Muslim nation,". "Sounds familiar?" "It is a crime against humanity."
ShrapnelA doctor in the town of Ajdabiya, Suleiman Refardi, told the BBC that on Wednesday, the coalition air strike on Zawia el Argobe a Government convoy targeted, the tanks, artillery and trucks contain ammunition.
A direct hit on an ammunition truck and trailer-a hail shrapnel into nearby homes sent, he said.
Four of the dead were female, including three children from the same family, between 12 and 16 years. Three boys aged between 14 and 20 years were also killed.
He said Dr. Refardi had spoken, the family of the girl, who had been slain and "was it not anger" with the coalition forces.
"When Ajdabiya had entered these tanks, it would have been a massacre," he said. "they [the Libyan people] are expecting more than this because they know the Gaddafi forces use civilians as a shield."
NATO officials told BBC that they made inquiries "down our operations chain all information to find out if indeed in the page operation that would support this claim".
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